HPLC Method for Analysis of Alachlor on Primesep 100 Column
https://sielc.com/hplc-method-for-alachlorChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode SIELC Technologies · HPLC Method for Analysis of Alachlor on Primesep 100 Column
Alachlor is a chloroacetanilide herbicide used to control annual grasses and broadleaf weeds primarily in corn and soybean crops. It is known for its pre-emergent activity, meaning it is applied to the soil to prevent weed germination and growth.
Alachlor be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm
Method Parameters
| Mobile Phase | MeCN – 55% |
|---|---|
| Buffer | H2SO4 -0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Samples | 1.0 mg/ml in MeCN – 100% |
| Injection volume | 1 µl |
| LOD* | 8 ppb (200 nm) |
| Class of Compounds | chloroacetanilide herbicides |
| Analyzing Compounds | Alachlor |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended