HPLC Determination of Lysine on Primesep 200 column in MS-compatible conditions
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Lysine , D-Lysine
Lysine is an essential amino acid used in the synthesis of proteins. In biological conditions, it is a basic, charged molecule. Lysine can be retained on Primesep 200 mixed-mode column which has embedded weak acidic ion-pairing groups for retention of basic analytes in HPLC. The mobile phase consisting of acetonitrile (ACN) and water with ammonium formate (AmFm) makes this method analysis MS-compatible.
Method Parameters
| Mobile Phase | MeCN/H2O – 10/90% |
|---|---|
| Buffer | AmFM pH 3.0 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona), MS- compatible phase |
| Class of Compounds | Acid, Hydrophilic, Ionizable, Carboxylic acid, Drug, Amino Acid |
| Analyzing Compounds | Lysine,D-Lysine |
HPLC Column Used
Primesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended