The Separation of Sodium, Dioctylsulfosuccinic Acid, and Sulfosuccinic Acid
https://sielc.com/Application-The-Separation-of-Sodium-Dioctyl-Sulfosuccinic-Acid-and-Sulfosuccinic-AcidChromatogram
Description
Sulfosuccinic acid and the corresponding dioctyl ester were separated on the Primesep B4 short carbon-chain anion-exchange column. Short carbon chain stationary phase allows to elute hydrophobic and acidic diester in reasonable time. No elution was observed on the mixed-mode columns with longer carbon chain analogs due to very strong hydrophobic and anion-exchange interaction.
Method Parameters
| Mobile Phase | Gradient MeCN – 10 -50%, 10 min |
|---|---|
| Buffer | Gradient AmFm pH 3.0- 60-180 mM, 10 min |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 50C |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Dioctyl Sulfosuccinic acid, Sodium Docusate |
HPLC Column Used
Primesep B4, 3.2×50 mm, 5 µm, 100A