Simultaneous Determination of Sodium and Sulfate Ions on Obelisc N Mixed-mode HILIC Column
https://sielc.com/Application-Simultaneous-Determination-of-Sodium-and-Sulfate-Ions-on-Obelisc-N-Mixed-mode-HILIC-ColumnChromatogram
Description
Inorganic cations and anions are usually analyzed by two different methods – cation-exchange and anion-exchange. This requires development and validation of two methods. Both ions can be analyzed on mixed-mode HILIC column with ELSD detection. Both ions are retained by combination of HILIC and ion-exchange mechanism.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmFm, pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Sulfate Ions |
HPLC Column Used
Obelisc N, 4.6×50 mm, 5 µm, 100A