Separation of Quaternary Amines
https://sielc.com/Application-Separation-of-Quaternary-AminesChromatogram
Description
Primesep C separates a mixture of quaternary amines by a combination of cation exchange, complex formation, and hydrophic interactions. Methyltriethylammonium, tetraethylammonium, and tetramethylammonium cations as well as bromide counter ion are separated on a short 50 mm column. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and triethylamine acetate with evaporative light scattering detection (ELSD).
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | TEA acetate |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Quaternary Amines, Ionizable, Hormone |
| Analyzing Compounds | Methyltriethylammonium, tetraethylammonium, Tetramethylammonium |
HPLC Column Used
Primesep C, 4.6×50 mm