Separation of Quaternary Amines by Reverse-Phase Mechanism
https://sielc.com/Application-Separation-of-Quaternary-Amines-By-Reverse-Phase-MechanismChromatogram
Description
Primesep B separates quaternary amines, such as t-butylamine with symmetrical peak shape by a combination of reversed-phase and ion-exclusion mechanisms. The embedded basic functional group on the stationary phase shields the underlying silanols to prevent peak tailing. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with evaporative light scattering detection (ELSD).
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | TFA |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Ions, Hydrophilic, Ionizable, Quaternary amines |
| Analyzing Compounds | t-butylamine |
HPLC Column Used
Primesep B, 4.6×150 mm, 5 µm, 100A