HPLC Separation of Acetylcholines
https://sielc.com/Application-Separation-of-AcetylcholinesChromatogram
Description
Primesep C separates acetylcholine and methylacetylcholine from their chloride counter ion with baseline resolution by a combination of cation exchange, complex formation, and hydrophobic interactions. The mass spec (LC/MS) compatible method uses a mobile phase of water, acetonitrile (MeCN, ACN) and ammonium acetate with evaporative light scattering detection (ELSD).
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmAc ph 4.1 |
| Flow Rate0.5 | ml/min |
| Detection | ELSD |
| Class of Compounds | Drug |
| Analyzing Compounds | Acetylcholine, Methylacetylcholine |
HPLC Column Used
Primesep C, 3.2×150 mm, 5 µm, 100A