Reverse Elution Order in Separation of Alkali Cation
https://sielc.com/Application-Reverse-Elution-Order-in-Separation-of-Alkali-CationChromatogram
Description
Primesep C separates a mixture of inorganic cations, potassium, sodium, and lithium, by a combination of cation exchange and complex formation. The peak order is reversed on the Primesep C column compared to the order normally obtained on columns that rely on cation exchange alone. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate is compatible with UV and evaporative light scattering detection (ELSD).
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmAc |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds | Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Sodium, Potassium, Lithium, Chloride |
HPLC Column Used
Primesep C, 4.6×50 mm, 5 µm, 100A