Primesep C Column Retention Control
https://sielc.com/Application-Primesep-C-Column-Retention-ControlChromatogram
Description
Primesep C separates benzylamines with an easily tunable HPLC method that allows retention control. Benzylamine, N-methylbenzylamine, and N,N-dimethylbenzylamine peak order and retention can be significantly changed by altering the water/acetonitrile ratio in the mobile phase which changes the combination of cation exchange, complex formation, and hydrophobic interactions. This mixture is separated with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 210 nm.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | TEAPh |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 |
| Class of Compounds | Hydrophilic, Ionizable |
| Analyzing Compounds | Benzylamine, N-methylbenzylamine, N,N-dimethylbenzylamine |
HPLC Column Used
Primesep C, 4.6×150 mm, 5 µm, 100A