Pirimicarb Analysis on Obelisc and Primesep Mixed-Mode HPLC Columns
https://sielc.com/Application-Pirimicarb-Analysis-on-Obelisc-and-Primesep-Mixed-Mode-HPLC-ColumnsChromatogram
Description
Pirimicarb is a carbamate pesticide used mostly to control aphids on non-food crops. The EURL included pirimicarb as a target pesticide for the EUPT-CF9. Obelisc R was used to retain pirimicarb and separate it from impurities. Primesep B2 and Obelisc R were used to retain pirimicarb and separate it from impurities. Primesep B2 contains embedded basic ion-pairing groups and Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing pirimicarb and dozens of other pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 4,5- 20-50 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Pirimicarb |
HPLC Column Used
Obelisc R, 2.1×150 mm, 5 µm, 100A