HPLC Retention Control of Antibiotics (Metronidazole and Norfloxacine) in Toothpaste
https://sielc.com/HPLC_Retention_Control_of_Antibiotics_in_ToothpasteChromatogram
Description
Commercially available toothpaste has been known to include antibiotics. To demonstrate the retention control on the mixed-mode column Primesep 100, toothpaste was spiked with the antibiotics metronidazole and norfloxacin. In a 13 minute method, retention of the antibiotics was controlled by adjustment in ratio of organic in the mobile phase. This method demonstrates the flexibility of using mixed-mode columns such as Primesep 100.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Antibiotics, Hydrophilic, Ionizable |
| Analyzing Compounds | Metronidazole, Norfloxacine |
HPLC Column Used
Primesep 100, 4.6×150 mm, 5 µm, 100A