HPLC Dye Separation on SHARC1 Column
https://sielc.com/HPLC_Dye_Separation_on_SHARC1_ColumnChromatogram
Method Parameters
| Mobile Phase | MeCN/EtOH – 99/1% |
|---|---|
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.5 ml/min |
| Detection | UV, 500, 650 nm |
| Class of Compounds | Dye, Hydrophilic, Ionizable |
| Analyzing Compounds | Methyl Red, Methyl Orange, Methylene Blue, Nile Blue A |
HPLC Column Used
Sharc 1, 3.2×100 mm, 3 µm, 100A