HPLC Analysis of Epinephrine and Epinephrine Sulfonate on Primesep AB Column
https://sielc.com/HPLC_Analysis_of_Epinephrine_and_Epinephrine_Sulfonate_on_Trimodal_ColumnChromatogram
Description
Epinephrine and epinephrine sulfonate were analyzed using Primesep AB, a reverse-phase column with both acidic and basic embedded ion-pairing groups. This method is LC/MS compatible.
Method Parameters
| Mobile Phase | MeCN/H2O – 5/95% |
|---|---|
| Buffer | H3PO4 – 0.25% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 280 nm |
| Class of Compounds | Drug, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Epinephrine, Epinephrine Sulfonate |
HPLC Column Used
Primesep AB, 4.6×250 mm, 5 µm, 100A