HPLC Separation of Sodium, Chloride, Bromide, and Iodine

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Chromatogram

Description

Halide ions are usually analyzed by ion-chromatography with a conductivity detector. Mixed-mode chromatography in combination with ELSD is offering a valuable alternative to ion-chromatography with conductivity detection. Chloride, bromide and iodide were separated on a Primesep SB mixed-mode anion-exchange column. Mobile phase is compatible with ELSD and LC/MS.

Method Parameters

Mobile PhaseMeCN/H2O
BufferAmFm
Flow Rate1.0 ml/min
DetectionELSD
Class of CompoundsHydrophilic, Ions
Analyzing CompoundsChloride, Sodium, Bromide, Iodine

HPLC Column Used

Primesep SB, 4.6×50 mm, 5 µm, 100A