HPLC Separation of Polar and Hydrophobic Drugs on Obelisc R
https://sielc.com/Application-HPLC-Separation-of-Polar-and-Hydrophobic-Drugs-on-Obelisc-R-and-NChromatogram
Method Parameters
| Mobile Phase | MeCN/H2O – 40/60% |
|---|---|
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 220 nm |
| Class of Compounds | Drug, Acid, Preservative, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | Propyl paraben, Dopamine, DOPA |
HPLC Column Used
Obelisc R, 4.6 x 150 mm, 5 µm, 100 A, dual ended