HPLC Separation of Lecithin and Soy lecithin on Primesep N Column
https://sielc.com/Application-HPLC-Separation-of-Lecithin-and-Soy-Lecithin-on-Primesep-N-ColumnChromatogram
Description
Lecithin is a naturally occurring compound composed of phosphoric acid, choline, fatty acids, glycerol, glycolipids, triglycerides and phospholipids.
Method Parameters
| Mobile Phase | Gradient MeCN 92-75%, 20 min |
|---|---|
| Buffer | Gradient AmFm pH 3.0- 8-20 mM, 20 min |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 40C |
| Class of Compounds | Aminoacid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Lecithin |
HPLC Column Used
Primesep N, 4.6×150 mm, 5 µm, 100A