HPLC Separation of Estrone
https://sielc.com/Application-HPLC-Separation-of-EstroneChromatogram
Description
Estrone, also known at oestrone and E1 is a naturally occurring estrogen which is the predominant estrogen in postmenopausal women. It can be found in its long-lived form estrone sulfate. In certain scenarios estrone can be a carcinogen and causes breast pain, nausea, headaches and leg cramps. It was separated on both Obelisc R and Primesep 100 which have unique modes of retention. Method is LC/MS compatible and useful for separating a number of pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Hormone, Hydrophobic, Ionizable |
| Analyzing Compounds | Estrone |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A