HPLC Separation of Epinephrine and Epinephrine Sulfonate
https://sielc.com/Application-HPLC-Separation-of-Epinephrine-and-Epinephrine-SulfonateChromatogram
Description
Epinephrine and epinephrine sulfonate are separated on combination of Primesep 100 and Primesep SB HPLC columns. Primesep 100 column retains epinephrine by combination of reversed-phase and cation-exchange mechanisms. Epinephrine sulfonate is retained by weak reversed-phase mechanism. Due to strong zwitter-ionic nature of epinephrine sulfonate, ionic interaction is not available for this molecule. Compounds can be monitored by UV, ELSD, CAD or LC/MS.
Method Parameters
| Mobile Phase | MeCN/H2O – 70/30% |
|---|---|
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Epinephrine, Epinephrine Sulfonate |
HPLC Column Used
Primesep SB, Primesep 100 4.6×250 mm, 5 µm, 100A