HPLC Separation of Dyes
https://sielc.com/Application-HPLC-Separation-of-DyesChromatogram
Description
Various dyes are widely used in food, textile, and pharmaceutical industries. Dies are aromatic compounds containing ionizable groups. Methylene blue, crystal violet lactone, and crystal violet were separated on a Primesep D HPLC column. Method can be used for separation of other dyes and pigments that are basic and hydrophobic in nature.
Method Parameters
| Mobile Phase | MeCN |
|---|---|
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 300 nm |
| Class of Compounds | Dye, Hydrophilic, Ionizable |
| Analyzing Compounds | Methylene Blue, Crystal violet lactone, Crystal violet |
HPLC Column Used
Primesep D, 4.6×50 mm, 3 µm, 100A