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☰ HPLC Applications by Compound
HPLC Separation of Dyes
https://sielc.com/Application-HPLC-Separation-of-Dyes
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Chromatogram
Method Parameters
Mobile Phase
MeCN
Buffer
H3PO4 – 0.2%
Flow Rate
0.5 ml/min
Detection
UV, 300 nm
Class of Compounds
Dye, Hydrophilic, Ionizable
Analyzing Compounds
Methylene Blue, Crystal violet lactone, Crystal violet
HPLC Column Used
Primesep D, 4.6×50 mm, 3 µm, 100A
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