HPLC Separation of Dopamine and Epinephrine in HILIC and Cation-Exchange Modes
https://sielc.com/Application-HPLC-Separation-of-Dopamine-and-Epinephrine-in-HILIC-and-Cation-Exchange-ModesChromatogram
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Description
Dopamine and epinephrine were separated on a Primesep S2 HILIC mixed-mode column. Dopamine and epinephrine are retained by HILIC and cation-exchange mechanisms. Retention time is controlled by the amount of ACN, buffer and buffer pH. Method is compatible with LC/MS and can be used for analysis of polar metabolites in biofluids.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Acid, Monocarboxylic acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | Dopamine, Epinephrine |
HPLC Column Used
Primesep S2, 4.6×150 mm, 5 µm, 100A