HPLC Separation of Chloride and Nitrate Ions
https://sielc.com/Application-HPLC-Separation-of-Chloride-and-Nitrate-IonsChromatogram
Description
High nitrate concentrations in water and soil can be toxic to humans, fish and domestic animals. Chloride can be used for analysis of water contamination. Primesep D column can be used to separate and quantify both ions. The baseline separation between chloride and nitrate can be increased by decreasing the ammonium formate concentration in the mobile phase. Evaporative Light Scattering Detection (ELSD) used.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Hydrophilic, Ion |
| Analyzing Compounds | Chloride, Nitrate |
HPLC Column Used
Primesep D, 4.6×150 mm, 5 µm, 100A