HPLC Separation of Chlorate and Bromate Ions
https://sielc.com/Application-HPLC-Separation-of-Chlorate-and-Bromate-IonsChromatogram
Description
Primesep B2 separates chlorate and bromate by an ion-exchange mechanism. The anion-exchange properties of the column allow retention of hydrophilic compounds, and these properties can be adjusted by simply changing the ammonium formate buffer concentration. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and ammonium formate with evaporative light scattering detection (ELSD).
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70% |
|---|---|
| Buffer | AmFm pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium Chlorate, Sodium Bromate |
HPLC Column Used
Primesep B2, 4.6×150 mm, 5 µm, 100A