HPLC Separation of Barium Cation in the Presence of Citric Acid, Sucrose and Tween 80
https://sielc.com/Application-HPLC-Separation-of-Barium-Cation-in-the-Presence-of-Citric-Acid-Sucrose-and-Tween-80Chromatogram
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Description
Barium ion and citric acid are analyzed in the presence of sucrose and Tween 80 on Obelisc N HILIC mixed-mode column. Both compounds are retained in ion-exchange mode and HILIC is not employed with this method. Obelisc N HPLC column has basic and acidic ion-exchange groups separated by long hydrophilic chain. Presence of that long chain allows both cation- and anion-exchange mechanisms to exist simultaneously. Obelisc N column can be used for analysis of cation and anions in HILIC/ion-exchange mode and in ion-exchange mode. Analytes can be monitored by ELSD/CAD and LC/MS.
Method Parameters
| Mobile Phase | MeCN |
|---|---|
| Buffer | AmAc pH 4.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Acid, Ion, Hydrophobic, Ionizable |
| Analyzing Compounds | Barium ion, Citric acid, Sucrose, Tween 80 |
HPLC Column Used
Obelisc N, 4.6×150 mm, 5 µm, 100A