HPLC Analysis of Xylitol on Primesep S Column
https://sielc.com/Application-HPLC-Analysis-of-Xylitol-on-Primesep-S-ColumnChromatogram
Description
Xylitol is a polyalcohol which is used as a sweetener for people with diabetes. Xylitol is naturally found in the fibers of fruits and vegetables. It is a very highly polar, non-ionizable molecule. Xylitol can be well retained on the Primesep S HILIC column. It has a relatively high water concentration in the mobile phase that helps to dissolve xylitol at high acetonitrile HILIC applications. Polar surface on the Primesep S column provides well controlled retention time and can be used as a robust method for analysis of various sugar-lie molecules.
Method Parameters
| Mobile Phase | MeCN/H2O – 80/20% |
|---|---|
| Buffer | AmFm pH 3.0- 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Sugar, Hydrophilic, Ionizable |
| Analyzing Compounds | Xylitol |
HPLC Column Used
Primesep S, 4.6×150 mm, 5 µm, 100A