HILIC Separation of Sugars on Obelisc N
https://sielc.com/Application-HILIC-Separation-of-Sugars-on-Obelisc-NChromatogram
Description
Common sugars can be separated on an Obelisc N HILIC/cation-exchange column with LC/MS compatible mobile phase. Highly polar surface of Obelisc N column provides good retention for sorbitol, sucrose, lactose and raffinose. Other sugars can be separated using this column.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Hydrophilic, Ionizable, Sugars |
| Analyzing Compounds | Sorbitol, Sucrose, Lactose, Raffinose |
HPLC Column Used
Obelisc N, 4.6×150 mm, 5 µm, 100A