Captan Retention on Obelisc R HPLC Column
https://sielc.com/Application-Captan-Retention-on-Obelisc-R-HPLC-ColumnChromatogram
Description
Captan is a phthalimide fungicide which is typically used as a component in industrial pesticide mixtures. The EURL included a QuEChERS (Quick, Easy, Cheap, Rugged, and Safe) method for captan as a target pesticide for EUPT-SRM. Obelisc R was used to retain captan and separate it from impurities. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing captan.
Method Parameters
| Mobile Phase | MeCN – 45% |
|---|---|
| Buffer | AmFm pH 3.0- 40 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Captan |
HPLC Column Used
Obelisc R, 4.6×150 mm, 5 µm, 100A