Azinphos-ethyl Analysis on Mixed-Mode HPLC Columns
https://sielc.com/Application-Azinphos-ethyl-Analysis-on-Mixed-Mode-HPLC-ColumnsChromatogram
Description
Azinphos-ethyl is a highly toxic organophosphate insecticide. Azinphos-ethyl was retained on both Obelisc R and Primesep B2 mixed-mode HPLC columns. Primesep B2 contains embedded basic ion-pairing groups while Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing azinphos-ethyl and other organophosphate insecticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Azinphos-ethy |
HPLC Column Used
Obelisc R, 2.1×150 mm, 5 µm, 100A