HPLC Analysis on Xylose and Xylitol on Primesep S Column
https://sielc.com/Appliccation-HPLC-Analysis-on-Xylose-and-Xylitol-on-Primesep-S-ColumnChromatogram
Description
Xylose is a monosaccharide which can be found in wood. Xylitol is a sugar-like, open chain polyalcohol. Xylitol and xylose were separated on the Primesep S HILIC/mixed-mode column. Both compounds are retained by a single HILIC mechanism. This method is compatible with ELSD, CAD, and RI detection and can be used for analysis of various monosaccharides in complex mixtures.
Method Parameters
| Mobile Phase | MeCN/H2O – 80/20% |
|---|---|
| Buffer | AmFm pH 3.0- 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Sugar, Hydrophilic, Ionizable |
| Analyzing Compounds | Xylose, Xylitol |
HPLC Column Used
Primesep S, 4.6×150 mm, 5 µm, 100A