Diphanamid Separation on Primesep B2 Column
https://sielc.com/Applicatino-Diphanamid-Separation-on-Primesep-and-Obelisc-Mixed-Mode-HPLC-ColumnsChromatogram
Description
Diphenamid is a pre-emergence herbicide used in production of tobacco, groundnuts, and tomatoes. Obelisc R and Primesep B2 were used to retain diphenamid and separate it from impurities. Primesep B2 contains embedded basic ion-pairing groups while Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing diphenamid and dozens of other pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 25-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 30-60 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Diphenamid |
HPLC Column Used
Primesep B2, 2.1×150 mm, 5 µm, 100A