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Chromatogram

Method Parameters

Mobile PhaseMeCN/H2O
BufferH2SO4 – 0.2 %
Flow Rate1.0 ml/min
DetectionUV, 215 nm
Class of CompoundsDrug, Basic, Hydrophobic, Ionizable, Zwitterionic
Analyzing Compoundsm-Xylylenediamine (MXDA)

HPLC Column Used

Primesep AP, 4.6×150 mm, 5 µm, 100A