Amino Sugars Analysis in Ion Exchange and Reverse Mode
https://sielc.com/Application-Amino-Sugars-Analysis-in-Ion-Exchange-and-Reverse-ModeChromatogram
Description
Primesep 100 retains an amino sugar by a combination of cation exchange and reversed phase. Columns with reversed-phase functionality alone are unable to retain this amino sugar. The polar interactions of the Primesep 100 column together with the reversed-phase interactions retain the amino sugar and resolve it from its many impurities. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and sulfuric acid (H2SO4) with UV detection at 205 nm.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | H2SO4 |
| Flow Rate | 1.5 ml/min |
| Detection | UV 205 nm |
| Class of Compounds | Hydrophilic, Ionizable |
| Analyzing Compounds | Sugars |
HPLC Column Used
Primesep 100, 4.6×150 mm, 5 µm, 100A