USP Methods for the Analysis of Naproxen Using Legacy L1 Column

Application Notes: Naproxen is a common NSAID used for pain relief and fever reducer. According to the USP methods, naproxen contains no less than 98% and no more than 101.5 percent naproxen calculated on a dried basis. The USP HPLC method for the separation of naproxen was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 µm and pore size of 100-120 Å. Resolution between critical pairs corresponds to rules and specifications of USP.

Application Columns: Legacy L1 C18HPLCcolumn

Application compounds: naproxen and benzophenone

Mobile phase: water/MeCN/Glacial Acetic acid (49/50/1)

Detection technique: UV

Reference: USP35: NF30