USP Method for the Analysis of Norepinephrine using the Legacy L1 Column
Application Notes: Norepinephrine is a naturally occurring hormone and neurotransmitter. The USP HPLC method for the separation of norepinephrine was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 µm and pore size of 100-120 Å. Resolution between critical pairs corresponds to rules and specifications of UPS.
Application Columns: Legacy L1 C18HPLCcolumn
Application compounds: Norepinephrine
Mobile phase: Sodium heptanesulfonate/H2O/MeOH0.22/160/40 pH 3.0
Detection technique: UV
Reference: USP35: NF30
Methodenparameter
| Column | Legacy L1, 4.6×250 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | Sodium heptanesulfonate/H2O/MeOH0.22/160/40 pH 3.0 |
| Buffer | Sodium heptanesulfonate |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 280 nm |