Low-UV Detection HPLC Method for PEI Analysis on PEI Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Polyethylenimine PEI
Polyethylenimine ( PEI ), also known as polyaziridine , is a polymer with the molecular formula (C2H5N) n . It has repeating units of the amine group and carbon aliphatic spacers. PEI has a large variety of uses. Most commonly, it is used in detergents, adhesives, water treatment agents, and cosmetics. Medically, due to being a catatonic polymer, it has been also studied as a gene delivery vehicle with successful outcomes.
Polyethylenimine PEI can be retained and analyzed using the PEI stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Methodenparameter
| Column | PEI, 4.6 x 250 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | 0.05% HClO4in MeCN/H2O – 20/80% |
| Flow Rate | 0.5 mL/min |
| Detection | UV 200 |