LCMS Method for Analysis of N-Acetylglucosamine-6-phosphate and N-Acetylglucosamine-1-phosphate on Newcrom B Column
N-Acetylglucosamine-6-phosphate (GlcNAc-6-P) and N-Acetylglucosamine-1-phosphate (GlcNAc-1-P) are two phosphorylated derivatives of N-acetylglucosamine (GlcNAc), a monosaccharide that is part of various biological structures, such as glycoproteins and glycosaminoglycans.
Both GlcNAc-6-P and GlcNAc-1-P are involved in the regulation of metabolism and glycosylation, but they differ in the position of the phosphate group on the glucose structure.
N-Acetylglucosamine-6-phosphate (GlcNAc-6-P) and N-Acetylglucosamine-1-phosphate (GlcNAc-1-P) can be retained, separated and analyzed using an Newcrom B mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid as a buffer. Detection is achieved using LC MS.
Methodenparameter
| Column | Newcrom B, 3.2 x 150 mm, 3 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | Formic Acid – 1.0% |
| Flow Rate | 0.5 mL/min |
| Detection | SIM300 -, SIM302 + |
| Sample | 0.5 mg/ml |
| Injection Volume | 1 µl |