HPLC Separation of Uracil, Thymine, Hypoxanthine and Guanine on Newcrom AH
Separation type: Liquid Chromatography Mixed-mode
Uracil, Thymine, Hypoxanthine, and Guanine are all nitrogenous bases, each playing distinct roles in the biochemistry of nucleic acids.
These nitrogenous bases are crucial for the structure, replication, and function of nucleic acids. Understanding their properties and interactions is fundamental to molecular biology and genetics.
Nucleotides can be retained and analyzed on a mixed-mode Newcrom AH column with a mobile phase consisting of water, Acetonitrile (MeCN), and ammonium formate. This analytical method can detect compounds with high resolution and peak symmetry using UV detection at 260 nm
Methodenparameter
| Column | Newcrom AH, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 1% |
| Buffer | Ammonium formate pH 3.0- 6 mM |
| Flow Rate | 1.0 mL/min |
| Detection | 260 nm |