HPLC Separation of Procymidone on Newcrom B Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of Procymidone .
Procymidone is a pesticide and fungicide with the chemical formula C13H11Cl2NO2 . It is typically used to kill unwanted ferns, nettles, and fungi in lupins, grapes, stone fruit, and strawberries.
Procymidone can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | Formic Acid – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 260 nm |