HPLC Separation of Phenylalanine, Glucosamine, and Tryptophan on Mixed-Mode Column
The Separation of Phenylalanine, Glucosamine and Tryptophan demonstrates how the small changes to the organic and/or buffer concentrations in the mobile phase affect the retention of compounds on a mixed-mode column by hydrophobic, ion-exchange and ion-exclusion mechanisms in reverse-phase HPLC chromatography. Evaporative Light Scattering Detector (ELSD) used
Methodenparameter
| Column | Primesep 100, 2.1×100 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm |
| Flow Rate | 0.25 mL/min |
| Detection | ELSD |