HPLC Separation of Octopamine and Synephrine on Primesep 200 Column
Octopamine is an analog of norepinephrine in invertebrates that’s an important neurotransmitter and hormone. Synephrine is another molecule that has a similar structure to norepinephrine and is a very strong agonist of octopamine receptors in invertebrates. Both compounds can be baseline separated with an isocratic method in 30/70 Acetonitrile (ACN) and water mobile phase with a 30mM Ammonium Formate (AmFm) buffer at pH 3. UV detection 270 nm.
Methodenparameter
| Column | Primesep 200, 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | AmFm pH 3.0- 30 mM |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 270 nm |