HPLC Separation of Neurotransmitters
The neurotransmitters dl-DOPA, creatinine, hydroxytryptophan (5-HTP), and serotonin are separated in less than 4 minutes on a short 50 mm Primesep 200 column. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm. Ion-pair reagents were not needed for retention of these polar, hydrophilic compounds, instead, a combination of ion-exchange and reversed-phase interactions were used.
Methodenparameter
| Column | Primesep 200, 4.6×50 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 80/20% |
| Buffer | TFA – 0.05% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 250 nm |