HPLC Separation of Lecithin and Soy lecithin on Primesep N Column
Lecithin is a naturally occurring compound composed of phosphoric acid, choline, fatty acids, glycerol, glycolipids, triglycerides and phospholipids.
Methodenparameter
| Column | Primesep N, 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | Gradient MeCN92-75%, 20 min |
| Buffer | Gradient AmFm pH 3.0- 8-20 mM, 20 min |
| Flow Rate | 1.0 mL/min |
| Detection | ELSD, 40C |