HPLC Separation of Inosine and Deoxyinosine
The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column . Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.
Methodenparameter
| Column | Sharc 1, 3.2×100 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/MeOH – 97/3% |
| Buffer | Formic Acid 0.1% , AmFm – 0.01% |
| Flow Rate | 1.0 mL/min |
| Detection | UV, 270 nm |