HPLC Separation of Growth Regulators (Paclobutrazol, Uniconazole, Daminozide) on Newcrom A Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Paclobutrazol , Uniconazole-P , Daminozide .

Daminozide is a plant growth regulator with the chemical formula C6H12N2O3 . It has a variety of alternate names including: aminozide, Alar, Kylar, SADH, B-995, B-nine, and DMASA. As a pesticide, works through inhibiting the production of gibberellins. It was typically used in trees that produced fruit for consumption, but was withdrawn due to concerns of it being a carcinogen. It is still occasionally used in ornamental plants.

Paclobutrazol (PBZ) is an organic fungicide with the chemical formula C15H20ClN3O . It works through inhibiting the production of gibberellins, which are plant growth hormones. Through reducing reducing internodal growth, it causes stronger stems, greater root growth, and earlier fruitset. It can also reduce frost sensitivity in plants.

Uniconazole is a plant growth regulator with the chemical formula C15H18ClN3O . As a pesticide, works through inhibiting the production of gibberellins, which are plant growth hormones. It is typically used in perennials and ornamental plants to ensure they are a marketable size before they bloom.

Paclobutrazol , Uniconazole-P , Daminozide can be retained and analyzed using the Newcrom A stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Formic Acid buffer. Detection is performed using CAD.