HPLC Separation of EDTA, Oxymetazoline and Benzyl Alcohol in Nasal Spray
High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA, Oxymetazoline and Benzyl Alcohol
Benzyl alcohol is found in over-the-counter medications, topical creams, lotions, shampoos, and facial cleansers as an antibacterial, preservative, and/or fungicide. Benzyl alcohol is a colorless liquid with a mild pleasant aromatic scent. Benzyl alcohol is found in many essential oils including jasmine, hyacinth, neroli, rose, and ylang-ylang. Oxymetazoline is a decongestant that shrinks blood vessels in the nasal passages. All active compounds of nasal spray can be separated in HPLC on a Newcrom BH mixed-mode column. The analytical method uses acetonitrile (ACN) gradient and water with sulfuric acid (H2SO4) as buffer and UV detected at 260 nm.
Methodenparameter
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN – 2- 70%, 10 min |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 260 nm |