HPLC Separation of Dyes
Various dyes are widely used in food, textile, and pharmaceutical industries. Dies are aromatic compounds containing ionizable groups. Methylene blue, crystal violet lactone, and crystal violet were separated on a Primesep D HPLC column. Method can be used for separation of other dyes and pigments that are basic and hydrophobic in nature.
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A , AH , B , and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Methodenparameter
| Column | Primesep D, 4.6×50 mm, 3 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 0.5 mL/min |
| Detection | UV, 300 nm |