HPLC Separation of Cytidine-5′-monophosphate (CMP) and 2′-Deoxycytidine 5′-Monophosphate (dCMP) on Newcrom B Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2'-Deoxycytidine 5'-Monophosphate , Cytidine Monophosphate .
Cytidine-5′-monophosphate is a nucleoside monophosphate with C9H14N3O8Pchemical formula. It is a fundamental building block for RNA and DNA as well as an intermediate in phospholipid synthesis.
2′-Deoxycytidine 5′-Monophosphate is a deoxyribonucleotide with C9H14N3O7Pchemical formula. It is crucial when it comes to DNA synthesis. dCMP is often used in genetic research, biotechnology, and development of pharmaceuticals with a focus on antiviral and anticancer drugs.
2'-Deoxycytidine 5'-Monophosphate , Cytidine Monophosphate can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Methodenparameter
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeOH/H2O – 80/20% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 270 nm |