HPLC MS Method for the Analysis of Cytokinins: A Phytohormone Class and Brassinazole on Primesep 200 Column
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
It looks like you’re listing a series of compounds, most of which are cytokinins or related plant hormones. Here’s a brief overview and classification for the compounds you mentioned:
N6-(Δ2-Isopentenyl)adenine (iP):
N6-Benzyladenine (BA):
trans-Zeatin , Kinetin , N6-(Δ2-Isopentenyl)adenine , Thidiazuron , 6-Benzylaminopurine , Brassinazole can be retained, separated and analyzed using a Primesep 200 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and formic acid as a buffer. This method allows for detection using UV 290, 265 nm.
You can find detailed UV spectra of trans-Zeatin , Kinetin , N6-(Δ2-Isopentenyl)adenine , Thidiazuron , 6-Benzylaminopurine , Brassinazole and information about its various lambda maxima by visiting the following link .
Methodenparameter
| Column | Primesep 200, 2.1 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN – 20%, 4 min than Gradient MeCN/H2O – 20-90% 16 min |
| Buffer | Formic Acid -0.2% |
| Flow Rate | 0.2 mg/ml |
| Detection | UV 290 nm, 265 nm ESISIM220+, 216+, 204+,226+, 328+, 221+ |