HPLC MS Method for Analysis of Procainamide on Primesep 200 Column
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
Procainamide is a medication that belongs to the class of antiarrhythmic drugs. It is used to treat certain types of irregular heartbeats (arrhythmias), particularly those associated with atrial and ventricular fibrillation or atrial flutter. Procainamide works by stabilizing the heart’s electrical activity and helping to restore a normal rhythm.
Procainamide blocks sodium channels in the heart, which helps to slow down the conduction of electrical impulses. By doing so, it can prevent abnormal electrical activity in the heart.
Procainamide can be retained and analyzed using a Primesep 200 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and ammonium formate as a buffer. This method allows for detection using UV at 280 nm
Methodenparameter
| Column | Primesep 200, 2.1 x 100 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Gradient MeCN30-60%, 10 min |
| Buffer | AmFm pH 3.0 – 10 mM |
| Flow Rate | 0.2 mL/min |
| Detection | UV 280 nm, SIM + 236 |
| Injection Volume | 5 µl |