HPLC Method for Separation Povidone-iodine (PVP-I) on PVP Column

HPLC-Applikation • Datum: 2025/07/14
HPLC Method for Separation Povidone-iodine (PVP-I) on PVP Column Chromatogramm

High Performance Liquid Chromatography (HPLC) Method for Analysis of Iodide , PVP

Polidone Iodide (Povidone-Iodine / PVP-I) Polidone Iodide, also known as Povidone-Iodine (PVP-I), is a widely used antiseptic made from iodine and polyvinylpyrrolidone (PVP). This combination allows for a slow, sustained release of iodine, enhancing antimicrobial effects while reducing irritation.

PVP (Polyvinylpyrrolidone): A water-soluble synthetic polymer that acts as:

It helps form a stable complex with iodine, improving its medical use.

Iodine: The active antimicrobial agent, effective against:

Slow release from the PVP complex ensures long-lasting, gentle antiseptic action.

Methodenparameter

Mobile PhaseStep gradient MeCN/H2O
BufferAmmonium Acetate pH 4.0 – 20 mM
Flow Rate0.4 mL/min
DetectionELSD
Limit of DetectionPVP2ppm