High Performance Liquid Chromatography (HPLC) Method for Analysis of Iodide , PVP
Polidone Iodide (Povidone-Iodine / PVP-I) Polidone Iodide, also known as Povidone-Iodine (PVP-I), is a widely used antiseptic made from iodine and polyvinylpyrrolidone (PVP). This combination allows for a slow, sustained release of iodine, enhancing antimicrobial effects while reducing irritation.
PVP (Polyvinylpyrrolidone): A water-soluble synthetic polymer that acts as:
It helps form a stable complex with iodine, improving its medical use.
Iodine: The active antimicrobial agent, effective against:
Slow release from the PVP complex ensures long-lasting, gentle antiseptic action.
| Mobile Phase | Step gradient MeCN/H2O |
|---|---|
| Buffer | Ammonium Acetate pH 4.0 – 20 mM |
| Flow Rate | 0.4 mL/min |
| Detection | ELSD |
| Limit of Detection | PVP2ppm |