HPLC Method for Separation PEI and PVP on PVP Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of PVP , Polyethylenimine PEI
PVP , Polyethylenimine PEI can be retained and analyzed using the PVP column. The analysis utilizes an step gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium acetate. Detection is performed using ELSD.
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Methodenparameter
| Column | PVP, 2.1 x 50 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | Step gradient MeCN/H2O |
| Buffer | Ammonium Acetate pH 4.0 – 20 mM |
| Flow Rate | 0.2 mL/min |
| Detection | ELSD |
| Limit of Detection | PVP0.5 ppm, PEI1.7 ppm |